DNA Replication Fidelity
Base selection by polymerase achieves ~10^-4 error rate
This paper provides detailed analysis of the molecular mechanisms ensuring DNA replication fidelity. It documents the multiple layers of error correction that together achieve extraordinary accuracy: base selection by polymerase (10^-4 error rate), proofreading (10^-2 reduction), and mismatch repair (~10^-3 further reduction), achieving overall fidelity of ~10^-10 errors per base pair.
For business applications, this reveals that high-fidelity replication requires multiple redundant quality control mechanisms, not just a single checkpoint. Organizations scaling through franchising or geographic expansion need analogous layered systems: selection (hiring), proofreading (training), and mismatch repair (ongoing audits).
Key Findings from Kunkel (2004)
- Base selection by polymerase achieves ~10^-4 error rate
- Proofreading reduces errors by ~10^-2
- Mismatch repair provides additional ~10^-3 reduction
- Combined fidelity achieves ~10^-10 errors per base pair