Biology of Business

Biofilms: Survival Mechanisms of Clinically Relevant Microorganisms

Rodney M. Donlan, J. William Costerton

Clinical Microbiology Reviews (2002)

TL;DR

Biofilm bacteria survive 10-1,000x antibiotic concentrations that kill individuals—collective defense through chemical coordination.

By Alex Denne

Landmark review establishing the scientific framework for understanding biofilm resistance—how bacteria collectively survive conditions that kill individuals. Cited thousands of times; foundational to both clinical microbiology and organizational resilience theory. Central to the book's treatment of collective defense and cultural entrenchment.

Key Findings from Donlan & Costerton (2002)

  • Biofilm bacteria exhibit 10-1000x greater antibiotic resistance than planktonic cells
  • Approximately 80% of chronic and recurrent human infections involve biofilms
  • Horizontal gene transfer occurs 16,000x more frequently in biofilms
  • Multiple resistance mechanisms compound: matrix barrier, microenvironment, slow growth, persisters
  • Persister cells enter dormant state surviving antibiotic exposure without genetic changes

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